aavs1 tre3g egfp (Addgene inc)
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aavs1 tre3g egfp
Aavs1 Tre3g Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aavs1+tre3g+egfp/AAVS1-TRE3G-EGFP+(Plasmid+%2352343)/pm41886980-12-63-70
Average 93 stars, based on 36 article reviews
Aavs1 Tre3g Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aavs1+tre3g+egfp/AAVS1-TRE3G-EGFP+(Plasmid+%2352343)/pm41886980-12-63-70
Average 93 stars, based on 36 article reviews
aavs1 tre3g egfp - by Bioz Stars,
2026-09
93/100 stars
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Transfection:Article Title: Drosophila MIC10b can polymerize into cristae-shaping filaments Article Snippet: .. Cells were transfected with AAVS1-TRE3G-MIC10-FLAG-T2A-EGFP ( , EMBOJ), AAVS1-TRE3G-DmMIC10-FLAG-T2A-EGFP, or Article Title: Drosophila MIC10b can polymerize into cristae-shaping filaments. Article Snippet: .. Cells were transfected with AAVS1-TRE3GMIC10-FLAG-T2A-EGFP (Stephan et al, 2020, EMBOJ), AAVS1-TRE3GDmMIC10-FLAG-T2A-EGFP, or Plasmid Preparation:Article Title: Splice modulators target PMS1 to reduce somatic expansion of the Huntington’s disease-associated CAG repeat Article Snippet: .. The Article Title: Generation and characterization of a human induced pluripotent stem cell line (hiPSC) expressing the rEstus voltage sensor under doxycycline induction. Article Snippet: .. Date archived/stock creation date 20.01.2025, p9 Cell line repository/bank https://hpscreg.eu/cell-line/UK Bi015-B-6 Ethics/GMO work approvals Ethics Committee, Medical Faculty, Rheinische Friedrich-WilhelmsUniversität (275/08) Disclaimers for Addgene/repositorysourced used recombinant DNA resources https://www.addgene.org/52341/, hAAVS1 TALEN Left was a gift from SuChun Zhang (Addgene plasmid # 52341; https://n2t.net/addgene:52341; RRID: Addgene_52341)https://www.addgene. org/52342/, hAAVS1 TALEN Right was a gift from Su-Chun Zhang (Addgene plasmid # 52342; https://n2t.net/addg ene:52342; RRID:Addgene_52342) https://www.addgene.org/52343/, Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo. Article Snippet: pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV-synjGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3Gwas amplified from AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343; http://n2t.net/addgene:52343; RRID:Addgene_52343) using following primers pair: 50-GTCGA Catgtctagactggacaagag-3’/50-ACGCGTttacccggggagcatgtcaa-3’. .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV-synjGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3Gwas amplified from Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo Article Snippet: Human H9 embryonic stem cells (WiCell) were routinely propagated on mitotically inactivated mouse embryonic fibroblasts in Knockout DMEM (Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacement (Thermo Fisher Scientific), 1X Non-essential Amino Acids (Thermo Fisher Scientific), 1X Penicillin/Streptomycin (Thermo Fisher Scientific), 1X 2-Mercaptoethanol (Merck), 2mM L-glutamine (Thermo Fisher Scientific). .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570 ; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV- syn -jGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489 ; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3G was amplified from Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: For lentiviral expression, the NLS-Reader1.0-eGFP was PCR amplified and cloned by Gibson assembly into AgeI- and EcoR1-linearized FUGW (a gift from David Baltimore (Addgene plasmid # 14883) . .. To knock-in inducible WT and T>A GFP-PST-LacI fusion proteins into the AAVS1 locus in U2OS 2-6-3 cells, we PCR amplified GFP and LacI from a GFP-LacI expression plasmid (a kind gift from Iain Cheeseman) 68 and codon-optimized WT and T>A PST repeat sequences and cloned them by Gibson assembly into MluI and Article Title: AMPK regulates phagophore-to-autophagosome maturation. Article Snippet: .. Plasmid construction and genome editing The GFP-LC3B reporter was generated by cloning the coding sequences of LC3B into Modification:Article Title: Splice modulators target PMS1 to reduce somatic expansion of the Huntington’s disease-associated CAG repeat Article Snippet: .. The Sequencing:Article Title: Splice modulators target PMS1 to reduce somatic expansion of the Huntington’s disease-associated CAG repeat Article Snippet: .. The Recombinant:Article Title: Generation and characterization of a human induced pluripotent stem cell line (hiPSC) expressing the rEstus voltage sensor under doxycycline induction. Article Snippet: .. Date archived/stock creation date 20.01.2025, p9 Cell line repository/bank https://hpscreg.eu/cell-line/UK Bi015-B-6 Ethics/GMO work approvals Ethics Committee, Medical Faculty, Rheinische Friedrich-WilhelmsUniversität (275/08) Disclaimers for Addgene/repositorysourced used recombinant DNA resources https://www.addgene.org/52341/, hAAVS1 TALEN Left was a gift from SuChun Zhang (Addgene plasmid # 52341; https://n2t.net/addgene:52341; RRID: Addgene_52341)https://www.addgene. org/52342/, hAAVS1 TALEN Right was a gift from Su-Chun Zhang (Addgene plasmid # 52342; https://n2t.net/addg ene:52342; RRID:Addgene_52342) https://www.addgene.org/52343/, Ligation:Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo. Article Snippet: pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV-synjGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3Gwas amplified from AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343; http://n2t.net/addgene:52343; RRID:Addgene_52343) using following primers pair: 50-GTCGA Catgtctagactggacaagag-3’/50-ACGCGTttacccggggagcatgtcaa-3’. .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV-synjGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3Gwas amplified from Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo Article Snippet: Human H9 embryonic stem cells (WiCell) were routinely propagated on mitotically inactivated mouse embryonic fibroblasts in Knockout DMEM (Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacement (Thermo Fisher Scientific), 1X Non-essential Amino Acids (Thermo Fisher Scientific), 1X Penicillin/Streptomycin (Thermo Fisher Scientific), 1X 2-Mercaptoethanol (Merck), 2mM L-glutamine (Thermo Fisher Scientific). .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570 ; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV- syn -jGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489 ; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3G was amplified from Amplification:Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo. Article Snippet: pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV-synjGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3Gwas amplified from AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343; http://n2t.net/addgene:52343; RRID:Addgene_52343) using following primers pair: 50-GTCGA Catgtctagactggacaagag-3’/50-ACGCGTttacccggggagcatgtcaa-3’. .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV-synjGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3Gwas amplified from Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo Article Snippet: Human H9 embryonic stem cells (WiCell) were routinely propagated on mitotically inactivated mouse embryonic fibroblasts in Knockout DMEM (Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacement (Thermo Fisher Scientific), 1X Non-essential Amino Acids (Thermo Fisher Scientific), 1X Penicillin/Streptomycin (Thermo Fisher Scientific), 1X 2-Mercaptoethanol (Merck), 2mM L-glutamine (Thermo Fisher Scientific). .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570 ; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV- syn -jGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489 ; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3G was amplified from Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: For lentiviral expression, the NLS-Reader1.0-eGFP was PCR amplified and cloned by Gibson assembly into AgeI- and EcoR1-linearized FUGW (a gift from David Baltimore (Addgene plasmid # 14883) . .. To knock-in inducible WT and T>A GFP-PST-LacI fusion proteins into the AAVS1 locus in U2OS 2-6-3 cells, we PCR amplified GFP and LacI from a GFP-LacI expression plasmid (a kind gift from Iain Cheeseman) 68 and codon-optimized WT and T>A PST repeat sequences and cloned them by Gibson assembly into MluI and Knock-In:Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: For lentiviral expression, the NLS-Reader1.0-eGFP was PCR amplified and cloned by Gibson assembly into AgeI- and EcoR1-linearized FUGW (a gift from David Baltimore (Addgene plasmid # 14883) . .. To knock-in inducible WT and T>A GFP-PST-LacI fusion proteins into the AAVS1 locus in U2OS 2-6-3 cells, we PCR amplified GFP and LacI from a GFP-LacI expression plasmid (a kind gift from Iain Cheeseman) 68 and codon-optimized WT and T>A PST repeat sequences and cloned them by Gibson assembly into MluI and Polymerase Chain Reaction:Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: For lentiviral expression, the NLS-Reader1.0-eGFP was PCR amplified and cloned by Gibson assembly into AgeI- and EcoR1-linearized FUGW (a gift from David Baltimore (Addgene plasmid # 14883) . .. To knock-in inducible WT and T>A GFP-PST-LacI fusion proteins into the AAVS1 locus in U2OS 2-6-3 cells, we PCR amplified GFP and LacI from a GFP-LacI expression plasmid (a kind gift from Iain Cheeseman) 68 and codon-optimized WT and T>A PST repeat sequences and cloned them by Gibson assembly into MluI and Expressing:Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: For lentiviral expression, the NLS-Reader1.0-eGFP was PCR amplified and cloned by Gibson assembly into AgeI- and EcoR1-linearized FUGW (a gift from David Baltimore (Addgene plasmid # 14883) . .. To knock-in inducible WT and T>A GFP-PST-LacI fusion proteins into the AAVS1 locus in U2OS 2-6-3 cells, we PCR amplified GFP and LacI from a GFP-LacI expression plasmid (a kind gift from Iain Cheeseman) 68 and codon-optimized WT and T>A PST repeat sequences and cloned them by Gibson assembly into MluI and Clone Assay:Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: For lentiviral expression, the NLS-Reader1.0-eGFP was PCR amplified and cloned by Gibson assembly into AgeI- and EcoR1-linearized FUGW (a gift from David Baltimore (Addgene plasmid # 14883) . .. To knock-in inducible WT and T>A GFP-PST-LacI fusion proteins into the AAVS1 locus in U2OS 2-6-3 cells, we PCR amplified GFP and LacI from a GFP-LacI expression plasmid (a kind gift from Iain Cheeseman) 68 and codon-optimized WT and T>A PST repeat sequences and cloned them by Gibson assembly into MluI and Generated:Article Title: AMPK regulates phagophore-to-autophagosome maturation. Article Snippet: .. Plasmid construction and genome editing The GFP-LC3B reporter was generated by cloning the coding sequences of LC3B into Cloning:Article Title: AMPK regulates phagophore-to-autophagosome maturation. Article Snippet: .. Plasmid construction and genome editing The GFP-LC3B reporter was generated by cloning the coding sequences of LC3B into |